Project
Binding

Part:BBa_K1378009:Design

Designed by: Zhang Zijian & Meng Liuyi   Group: iGEM14_Peking   (2014-10-06)


INPNC-Linker (rTEV)-MVN & GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1528
    Illegal NheI site found at 1551
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 466
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2229
    Illegal SapI site found at 1071


Design Notes

We optimized the sequence of MVN beforehand and chose two different promoters to avoid possible homologous recombination. To ensure the normal functioning of INPNC and MVN we also added a 10aa flexible protein domain linker in between.


Source

This is a composite part so we constructed it using various different smaller parts. INPNC comes from the existing part BBa_K523013. The sequence of MVN can be found in the genomic sequence of Microcystis aeruginosa PCC7806 and the gene was synthesized by a company (sequence can be found in the part BBa_K1378003). GFP is common and comes from the existing part BBa_E0040. The amino acid sequence of the linker can be easily found in papers.

References